Phospho-EGFR (Tyr1068) (E5) rabbit mAb

• Catalog Number: orb1946283
• Category: Antibodies
• Description: Phospho-EGFR (Tyr1068) (E5) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human phospho EGFR.
• UniProt ID: P00533
• Tested applications: FC
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat and other homologues.
• Clone Number: EGFRY1068-E5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: avian erythroblastic leukemia viral (v-erb-b) onco
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of C6 cells secondary antibody only (blue) treated with imatinib (grey) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Flow cytometric analysis of HeLa cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Flow cytometric analysis of 3T3 cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 at 0.01μg/mL. Cat. #orb1946283.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF and pervanadate (green) using Phospho-EGFR (Tyr1068) antibody at 0.01 μg/mL EGFRY1068-E5. Cat. #orb1946283.

Flow cytometric analysis of 3T3 cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (Cat. #orb1946283) or Company C antibody at 0.5ug/mL (suggested concentration by the manufacturer).