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Catalog Number | orb1946283 |
---|---|
Category | Antibodies |
Description | Phospho-EGFR (Tyr1068) (E5) rabbit mAb Antibody |
Clonality | Monoclonal |
Clone Number | EGFRY1068-E5 |
Tested applications | FC |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgGk |
Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human phospho EGFR. |
Concentration | 0.5 mg/ml |
Dilution range | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
Conjugation | Unconjugated |
UniProt ID | P00533 |
Storage | -20°C |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | avian erythroblastic leukemia viral (v-erb-b) onco Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of C6 cells secondary antibody only (blue) treated with imatinib (grey) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.
Flow cytometric analysis of HeLa cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.
Flow cytometric analysis of 3T3 cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (green) Cat. #orb1946283.
Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 at 0.01μg/mL. Cat. #orb1946283.
Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF and pervanadate (green) using Phospho-EGFR (Tyr1068) antibody at 0.01 μg/mL EGFRY1068-E5. Cat. #orb1946283.
Flow cytometric analysis of 3T3 cells secondary antibody only (blue) treated with imatinib (grey) or pervanadate (orange) with 0.1 μg/mL of isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using 0.1 μg/mL of Phospho-EGFR (Tyr1068) antibody EGFRY1068-E5 (Cat. #orb1946283) or Company C antibody at 0.5ug/mL (suggested concentration by the manufacturer).
FC | |
Human, Mouse, Rat | |
Monoclonal | |
SureLight 488 |
FC | |
Human, Mouse, Rat | |
Monoclonal | |
APC |
FC | |
Human, Mouse, Rat | |
Monoclonal | |
FITC |
FC | |
Human, Mouse, Rat | |
Monoclonal | |
PE |
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