Phospho-CrkL (Tyr207) (G4) rabbit mAb

• Catalog Number: orb1946278
• Category: Antibodies
• Description: Phospho-CrkL (Tyr207) (G4) rabbit mAb
• Clonality: Monoclonal
• Clone Number: CrkLY207-G4
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: P46109
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Crk-like protein; CRKL; v-crk sarcoma virus CT10 o
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of K562 cells treated with imatinib (red) or treated with pervanadate (green) using Phospho-CRKL (Tyr207) (G4) Rabbit mAb CRKLY207-G4 #orb1946278 at 0.05 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with imatinib (black) or treated with pervanadate (blue).

Western blot analysis of K562 cell extract untreated or treated with imatinib using 1ng/mL CrkL (Tyr207) antibody CrkLY207-G4. Cat. #orb1946278.

Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-CrkL (Tyr207) antibody CrkLY207-G4 at 0.01μg/mL. Cat. #orb1946278.

Western blot analysis of NIH3T3 cell extract using 1μg/mL Phospho-CrkL (Tyr207) antibody CrkLY207-G4. Cat. #orb1946278.

Western blot of E10.5 mouse wild-type (+/+) or Crkl knock out (-/-) whole embryos. The red channel was stained using a β-actin loading control and the green channel was stained using 1:500 dilution of Phospho-CrkL (Tyr207) antibody CrkLY207-G4 Cat. #orb1946278. Phospho CrkL antibody staining is absent in the knock out lysate.

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 μg/mL of Phospho-CrkL (Tyr207) antibody CrkLY207-G4 (Cat. #orb1946278) or Company C antibody at 0.1ug/mL.