Phospho-Chk2 (Thr68) (D12) rabbit mAb Antibody

• Catalog Number: orb1946260
• Category: Antibodies
• Description: Phospho-Chk2 (Thr68) (D12) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: Chk2T68-D12
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human phospho Chk2
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: O96017
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Serine/threonine-protein kinase Chk2, Cds1 homolog
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Hela cells treated with lambda phosphatase (red) or treated with UV + TPA (green) using Phospho-CHK2 (Thr68) (D12) Rabbit mAb, at 0.01 ug/mL, CHK2T68-D12 #orb1946260, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #orb1946241 for cells treated with lambda phosphatase (black) or treated with UV + TPA (blue).

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or untreated (red) or UV/TPA-treated (green) or untreated and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-Chk2 (T68) antibody Chk2T68-D12 0.1μg/mL. Cat. #orb1946260.

Chk2T68-D12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-Chk2 (T68) antibody Chk2T68-D12 (green) Cat. #orb1946260.

Western blot analysis of HEK293 cell extract untreated or treated with UV using Phospho Chk2 (T68) antibody Chk2T68-D12. Cat. #orb1946260.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 μg/mL Phospho-Chk2 (T68) antibody Chk2T68-D12 (Cat. #orb1946260) or Company C antibody at 2.3 μg/mL (manufacturer's recommended concentration).