Phospho-CDKN1B (Ser178) Rabbit Polyclonal Antibody

• Catalog Number: orb704518
• Category: Antibodies
• Description: Phospho-CDKN1B (Ser178) Rabbit Polyclonal Antibody
• Target: CDKN1B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CDKN1B around the phosphorylation site of Ser178 DG(p-S)PN
• UniProt ID: P46527
• MW: 22 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: p27Kip1(phospho-Ser178); p-p27Kip1(Ser178); p-p27K
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1B (Ser178)) Polyclonal Antibody, Unconjugated (orb704518) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-CDKN1B (Ser178)) Polyclonal Antibody, Unconjugated (orb704518) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 2: Spleen (Rat) Lysate at 40 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 6: THP-1 (Human) Cell Lysate at 30 ug, Lane 7: HepG2 (Human) Cell Lysate at 30 ug, Lane 8: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-CDKN1B (Ser178) (orb704518) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-CDKN1B (Ser178) Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.