Phospho-CDK7(T170) Antibody

• Catalog Number: orb1931280
• Category: Antibodies
• Description: Phospho-CDK7(T170) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P50613
• MW: 39038 Da
• Tested applications: DOT, IHC-P, WB
• Dilution range: WB - 1:1000, DB - 1:500, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CAK, CAK1, CDKN7, MO15, STK1
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only

Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of extracts from Hela and MCF-7 cells, untreated or lamda phosphatase-treated, using Phospho-CDK7 (T170) Antibody (upper) or Beta-actin (lower).