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CDC25B Antibody
Description
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | WB: 1:500-1:1000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B . |
| Target | CDC25B |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Disclaimer | For research use only |
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Protocol Information
Yang, Eun Jae et al. Co-inhibition of ATM and ROCK synergistically improves cell proliferation in replicative senescence by activating FOXM1 and E2F1 Commun Biol, 5, 702 (2022)
CDC25B Antibody (orb571847)
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