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Phospho-C-Myc (Thr58+Ser62) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb526687
| Catalog Number | orb526687 |
|---|---|
| Category | Antibodies |
| Description | Phospho-C-Myc (Thr58+Ser62) Recombinant Rabbit Monoclonal Antibody |
| Target | MYC |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human c-Myc around the phosphorylation site of Thr58 and Ser62 LP(p-T)PPL(p-S)PS |
| UniProt ID | P01106 |
| MW | 49 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:300-500, IHC-P=1:100-500, IHC-F=1:20-200, ICC/IF=1:20-200, IF=1:20-200 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 2B11 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Myc(phospho-Thr58+Ser62); Myc(phospho-T58+S62); p- Read more... |
| Note | For research use only |
Western blot analysis of Phospho-c-Myc (T58+S62) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (orb526687, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate, Lane 2: SH-SY5Y cell lysate.
