Phospho-c-Fos (Ser32) rabbit mAb Antibody

SKU: orb1946027

Description

c-FOS belongs to the Fos family of nuclear oncogenes which include Fos B, Fos-related antigen 1 (FRA1), Fos-related antigen 2 (FRA2) in addition to c-Fos(1). Activator Protein-1 (AP-1) is formed upon dimerization of Fos proteins with Jun protiens (c-Jun, Jun B, and JunD)(2,3). AP-1 is considered a transcription factor that binds to TRE/AP-1 elements and activates transcription. ERK5 is involved with c-Fos phosphorylation at Ser32 and Thr232 which increase c-Fos stability and its nuclear translocation.

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BDC1
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser32 of human phospho c-Fos
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7

Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 μg/mL. Cat. #orb1946027.

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with UV + TPA (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.01μg/mL. Cat. #orb1946027.

Flow cytometric analysis of L929 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 μg/mL. Cat. #orb1946027.

Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV + TPA (green) using Phospho-c-Fos (Ser32) antibody cFosS32-BA9 at 0.001 μg/mL. Cat. #orb1946027.

Quick Database Links

UniProt

P01100