Phospho-c-Cbl (Tyr700) (E1) rabbit mAb Antibody

SKU: orb1946105

Description

The c-Cbl (Casitas B-lineage Lymphoma) proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that contains multiple functional domains, including an amino-terminal tyrosine kinase-binding (TKB) domain, a RING finger motif, and a proline-rich region. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) and on other nonreceptor tyrosine kinases, while the RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (1). The proline-rich region contains 14-3-3 protein-binding and SH3 domain-binding motifs. c-Cbl is phosphorylated at Y700, Y731, and Y774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for immunoglobulins, antigens, hormones, growth factors, and cytokines. Phosphorylated Y774 interacts with the SH2 domain of Crk (1,2). The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells (3,4). Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5).

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: CblY700-E1
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr700 of human phospho c-Cbl
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase, Signal transduction protein CBL, CBL2, RNF55

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 μg/mL isotype control Cat. #orb1946241 or imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 μg/mL. Cat. #orb1946105.

Peptide blocking flow cytometric analysis of C6 cells, secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 μg/mL. Cat. #orb1946105.

Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 μg/mL isotype control Cat. #orb1946241, or imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 μg/mL. Cat. #orb1946105.

Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.01 μg/mL. Cat. #orb1946105.

Quick Database Links

UniProt

P22681