Phospho-Btk (Tyr551) (G12) rabbit mAb Antibody

• Catalog Number: orb1946087
• Category: Antibodies
• Description: Phospho-Btk (Tyr551) (G12) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: BtkY551-G12
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Rabbit IgGk
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr551 of human phospho Btk
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q06187
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Bruton tyrosine kinase, Tyrosine-protein kinase BT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Btk (Tyr551) antibody BtkY551-G12 at 0.1 μg/mL. Cat. #orb1946087.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Btk (Y551) antibody BtkY551-G12 (Cat. #orb1946087) or Company B antibody at 0.1 μg/mL (manufacturer's recommended concentration).

Peptide ELISA using BtkY551-G12 (Cat. #orb1946087) titered starting from 0.3 ug/mL shows binding to only Btk pY551 phospho peptide and no cross-reactivity to Btk Y551 non-phospho peptide or to control peptides.

Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.25 mg/mL total protein coated lysate and 1 ug/mL BtkY551-G12 (Cat. #orb1946087) IgG.