Phospho-BLNK (Tyr84) (H4) rabbit mAb Antibody

SKU: orb1946129

Description

BLNK protein, known as SLP-65 play an important role as adaptor protein in B-lineage cells. BLNK associates with proteins in the cytoplasmic side of plasma membrane through its N-terminal leucine zipper motif. Upon BLNK activation on its tyrosine, BLNK binds to Btk, Vav, Brb2, Syk, and HPK1. Through this associations, BLNK mediates Ca2+ mobilization, for ERK1/2, JNK and p38 MAP kinase activation. After phosphorylation, BLNK binds Btk and PLCg2 through their SH2 domains and mediates PLCg2 activation by Btk. BLNK also binds other signaling molecules such as Vav, Grb2, Syk, and HPK1. BLNK plays aa important role in BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

Images & Validation

• Tested Applications: FC
• Dilution Range: 1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BLNKY84-H4
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr84 of human phospho BLNK
• Purification: Protein A+G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B-cell linker protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP65, B-cell adapter containing a SH2 domain protein, BASH

Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 μg/mL. Cat. #orb1946129.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 μg/mL. Cat. #orb1946129.

Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 10 ng/mL of Phospho-BLNK (Tyr84) antibody BLNKY84-H4 (Cat. #orb1946129) or Company B antibody at 2.5 ug/mL (manufacturer's recommended concentration).

Quick Database Links

UniProt

Q8WV28