Phospho-BAD (Ser112) (B9) rabbit mAb Antibody

• Catalog Number: orb1946164
• Category: Antibodies
• Description: Phospho-BAD (Ser112) (B9) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: BADS112-B9
• Tested applications: FC
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgGL
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser112 of human phospho BAD
• Concentration: 0.5 mg/ml
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Conjugation: Unconjugated
• UniProt ID: Q92934
• Storage: -20°C
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Alternative names: Bcl2-associated agonist of cell death, Bcl-2-bindi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using Phospho-BAD (Ser112) antibody BADS112-B9 at 0.1 μg/mL. Cat. #orb1946164.

BADS112-B9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or of Phospho-BAD (Ser112) antibody BADS112-B9 (green) Cat. #orb1946164.

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using Phospho-BAD (Ser112) antibody BADS112-B9 at 0.01μg/mL. Cat #orb1946164.

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using Phospho-BAD (S112) antibody BADS112-B9 (Cat. #orb1946164) or Company C antibody at 0.08 μg/mL (manufacturer's recommended concentration).