Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb FITC conjugate Antibody

SKU: orb1946050

Description

Aurora kinases (serine/threonine kinases) are essential requirement for the onset and progression of mitosis. These kinases share a similar protein structure as well as kinase activity, however each kinase display distinct cellular and subcellular localization. Each Aurora member is phosphorylated at specific residues upon co-factor binding during mitosis. Aurora kinases acquire active kinase conformations due to the activation loop. The active kinase conformation is acquired upon auto-phosphorylation through an intermolecular (trans)-reaction within Aurora kinase domain. Aurora Kinase A (Aurora A) is involved in G2/M transition. AuroraA promotes centrosome maturation and mitotic spindle assembly, whereas AuroraB and AuroraC act as chromosome-passenger complex proteins. They play a crucial role in chromosomal binding to kinetochores and segregation of chromosomes. Aurora B is widely distributed in the cell, while AuroraC is expressed mainly in the meiotically-active germ cells. Aurora kinases are auto-phosphorylated into active forms at conserved threonine residues (i.e. the Thr288 (AurA), Thr232 (AurB) and Thr195 (AurC) residues). AuroraA auto-phosphorylation is initiated by several co-factors acting at different steps of mitosis. AroraB and AruroaC auto-phosphorylation are mediated by survivin and Borealin proteins.

Images & Validation

• Tested Applications: FC
• Dilution range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: AuroraABC-CC12
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
• Purification: Protein A+G
• Conjugation: FITC

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Disclaimer: For research use only

Alternative Names

Aurora kinase A/B/C, AURKA, AURKB, AURKC

Flow cytometric analysis of HeLa cells untreated and unstained as negative control (blue) or untreated (red) or treated with nocodazole (green) and stained using Phospho-Aurora A (Thr288) /Aurora B (Thr232) /Aurora C (Thr198) antibody FITC conjugate, AuroraABC-CC12. Cat. #orb1946050.

Quick Database Links

UniProt

Q96GD4, O14965, Q9UQB9