Phospho-ATF2 (Thr71) (G3) rabbit mAb

• Catalog Number: orb1946307
• Category: Antibodies
• Description: Phospho-ATF2 (Thr71) (G3) rabbit mAb
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: Protein A+G
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human phospho ATF2
• UniProt ID: P15336
• Tested applications: FC, WB
• Dilution range: 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Cross Reactivity: Predicted to work with mouse, rat and other homologues.
• Clone Number: ATF2T71-G3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cyclic AMP-dependent transcription factor ATF-2, A
• Note: For research use only

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat #orb1946307.

Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 μg/mL Phospho-ATF-2 (Thr71) antibody ATF2T71-G3. Cat. #orb1946307.

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat. #orb1946307.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1μg/mL. Cat #orb1946307.

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (T71) antibody ATF2T71-G3 (Cat. #orb1946307) or Company C antibody at 0.07 μg/mL (manufacturer's recommended concentration).