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| Catalog Number | orb1563453 |
|---|---|
| Category | Antibodies |
| Description | Phospho-AMPK alpha 1/2 (Thr183/Thr172) Rabbit pAb |
| Target | AAPK1/AAPK2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Monkey, Mouse, Porcine, Rat |
| Form/Appearance | Liquid |
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Purification | Affinity Chromatography |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. |
| UniProt ID | Q13131, P54646 |
| MW | Calculated MW: 64 kDa; Observed MW: 64 kDa |
| Tested applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | PRKAA1; AMPK1; 5' -AMP-activated protein kinase ca Read more... |
| Research Area | Signal Transduction |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in various lysates using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody.

Western blot analysis of Phospho-AMPK alpha 1/2 (Thr183/Thr172) in KB lysates using Phospho-AMPKα1/2 (T183/17, 2) antibody.

Western blot analysis of Phospho-AMPK alpha 1/2 in rat muscle heart using Phospho-AMPK alpha 1/2 antibody.

Western blot analysis of Phospho-AMPK alpha 1/2 in rat muscle lysates using Phospho-AMPK alpha 1/2 antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-AMPK alpha 1/2 (Thr183/Thr172) antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody. was preabsorbed by immunogen peptide.

IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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