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Catalog Number | orb559194 |
---|---|
Category | Antibodies |
Description | Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody |
Target | PRKAA1 |
Clonality | Recombinant |
Species/Host | Rabbit |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Mouse, Rat |
Form/Appearance | Liquid |
Concentration | 1mg/ml |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from mouse AMPK alpha 1 around the phosphorylation site of Ser496 SG(p-S)VS |
UniProt ID | Q5EG47 |
MW | 64 kDa |
Tested applications | ICC, WB |
Dilution range | WB=1:500-2000, ICC/IF=1:50-200 |
Antibody Type | Recombinant Antibody |
Clone Number | 11G1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | p-AMPK alpha 1(S496); phospho-AMPK alpha-1(S496); Read more... |
Note | For research use only |
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser496) (orb559194) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.