Phospho-ABL1(Y251) Antibody

• Catalog Number: orb1931304
• Category: Antibodies
• Description: Phospho-ABL1(Y251) Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P00519
• MW: 122873 Da
• Tested applications: DOT, IF, IHC-P, WB
• Dilution range: DB - 1:500, WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ABL, JTK7
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot blot analysis of anti-Phospho-ABL1-Y251 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

Confocal immunofluorescent analysis of Phospho-ABL1-Y251 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-ABL Pab in Hela cell line lysate (35 ug/lane). ABL (arrow) was detected using the purified Pab.