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Catalog Number | orb629888 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PHLPP |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | PH domain and leucine rich repeat protein phosphatase |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
Target | PHLPP1 |
UniProt ID | O60346 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
mouse brain tissue were subjected to SDS PAGE followed by western blot with orb629888 (PHLPP antibody) at dilution of 1:1000.
Immunofluorescent analysis of HepG2 cells, using PHLPP antibody orb629888 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PHLPP (IP: orb629888, 4 ug; Detection: orb629888 1:1000) with mouse brain tissue lysate 5000 ug.
Immunohistochemistry of paraffin-embedded human liver slide using orb629888 (PHLPP Antibody) at dilution of 1:50.
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |