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Catalog Number | orb1929455 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB03701/03702 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 46442 Da |
Target | This PHKG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the Central region of human PHKG2. |
UniProt ID | P15735 |
NCBI | NP_000285.1, NP_001165903.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Phosphorylase b kinase gamma catalytic chain, live Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PHKG2 Antibody (G319) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).
PHKG2 Antibody (G319) western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).
Anti-PHKG2-G319 at 1:1000 dilution + 293T/17 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.