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Item 1 of 3
PHB2 Antibody (Y128)
Catalog Number: orb1929409
| Catalog Number | orb1929409 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from human PHB2. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q99623 |
| MW | 33296 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB15314 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Prohibitin-2, B-cell receptor-associated protein B Read more... |
| Note | For research use only |
| NCBI | NP_001254629.1, NP_001138303.1 |
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (Y128), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PHB2-Y128 in mouse NIH-3T3 cell line lysates (35 ug/lane).PHB2 (arrow) was detected using the purified Pab.
All lanes: Anti-Phospho-PHB2-Y128. ctrl at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
