PHB Antibody

SKU: orb1260377

Description

PHB Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human PHB (NP_002625.1).
• Target: PHB
• Molecular Weight: Observed: 30kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PHB, PHB1, HEL-215, HEL-S-54e

Western blot analysis of extracts of various cell lines, using PHB antibody (orb1260377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using PHB antibody (orb1260377). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

PHB

UniProt

P35232