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Item 1 of 5
PHB Antibody (Center)
Catalog Number: orb1931542
| Catalog Number | orb1931542 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P35232 |
| MW | 29804 Da |
| Tested applications | FC, IF, IHC-P, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB17513 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Prohibitin, PHB |
| Note | For research use only |
| NCBI | NP_001268425.1, NP_002625.1, NP_001268644.1 |
PHB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of PHB Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PHB Antibody (Center) in Hela cell line lysates (35 ug/lane). PHB (arrow) was detected using the purified Pab.
Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
