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PHB Antibody (Center)

Catalog Number: orb1931542

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1931542
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB17513
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW29804 Da
TargetThis PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB.
UniProt IDP35232
NCBINP_002625.1, NP_001268644.1, NP_001268425.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesProhibitin, PHB
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NoteFor research use only
Expiration Date12 months from date of receipt.
PHB Antibody (Center)

PHB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PHB Antibody (Center)

Confocal immunofluorescent analysis of PHB Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

PHB Antibody (Center)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PHB Antibody (Center)

Western blot analysis of PHB Antibody (Center) in Hela cell line lysates (35 ug/lane). PHB (arrow) was detected using the purified Pab.

PHB Antibody (Center)

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.