PHB Antibody (Center)

SKU: orb1931542

Description

PHB Antibody (Center)

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: IHC-P - 1:100-500, WB - 1:1000, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Bovine, Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 29804 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

PHB Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of PHB Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of PHB Antibody (Center) in Hela cell line lysates (35 ug/lane). PHB (arrow) was detected using the purified Pab.

Western blot analysis of PHB (arrow) using rabbit polyclonal PHB Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.

Quick Database Links

UniProt

P35232