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Catalog Number | orb11270 |
---|---|
Category | Antibodies |
Description | Prohibitin Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Equine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human Prohibitin (171-272/272aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=2ug/test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 30 kDa |
Target | PHB |
UniProt ID | P35232 |
RRID | AB_10747118 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Mitochondrial Marker; PHB; PHB1; Prohibitin-Mitoch Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (orb11270) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Sample: A431 (Human) Cell Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.
Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
ICC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, WB | |
Bovine, Equine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |