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Prohibitin Rabbit Polyclonal Antibody

Catalog Number: orb11270

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11270
CategoryAntibodies
DescriptionProhibitin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine, Equine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Prohibitin (171-272/272aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=2ug/test
Form/AppearanceLiquid
ConjugationUnconjugated
MW30 kDa
TargetPHB
UniProt IDP35232
RRIDAB_10747118
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesMitochondrial Marker; PHB; PHB1; Prohibitin-Mitoch
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NoteFor research use only
Expiration Date12 months from date of receipt.
Prohibitin Rabbit Polyclonal Antibody

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (orb11270) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Prohibitin Rabbit Polyclonal Antibody

Sample: A431 (Human) Cell Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Prohibitin Rabbit Polyclonal Antibody

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Prohibitin (orb11270) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 30 kD.

Prohibitin Rabbit Polyclonal Antibody

Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Prohibitin Rabbit Polyclonal Antibody

Tissue/Cell: Rat intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated (orb11270) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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