PGR/PR Antibody (C-term)

SKU: orb166115

Description

Rabbit polyclonal antibody to PGR (C-term).

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF/ICC: 1:10-50, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB41081
• Immunogen: Synthetic Peptide
• Target: PGR
• Molecular Weight: 98981
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti PGR antibody, anti NR3C3 antibody, anti Progesterone receptor antibody, anti Nuclear receptor subfamily 3 group C member 3 antibody

PGR/PR Antibody (C-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the PGR/PR antibody detected the PGR/PR protein (arrow).

Fluorescent image of U251 cell stained with PGR/PR Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PGR/PR primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).PGR/PR immunoreactivity is localized to Nucleus significantly and Vesicles weakly.

Quick Database Links

Gene Symbol

PGR

UniProt

P06401

RefSeq (Protein):NP_001258091.1, NP_001189403.1, NP_000917.3