PGP9.5 / UchL1 Antibody

• Catalog Number: orb699720
• Category: Antibodies
• Description: This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinsons disease.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant PGP9.5 antibody.
• UniProt ID: P09936
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant PGP9.5 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: UCHL1/4556R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone UCHL1/4556R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum with recombinant PGP9.5 antibody (clone UCHL1/4556R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain lysate with recombinant PGP9.5 antibody. Predicted molecular weight ~25 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant PGP9.5 antibody (clone UCHL1/4556R) as confirmation of integrity and purity.