PGP9.5 / UchL1 Antibody

• Catalog Number: orb1151552
• Category: Antibodies
• Description: Mouse monoclonal antibody to PGP9.5
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UCHL1/4558
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1 was used as the immunogen for the PGP9.5 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PGP9.5 antibody is available for research use only.
• UniProt ID: P09936
• Storage: Aliquot the PGP9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PGP9.5 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum tissue with PGP9.5 antibody (clone UCHL1/4558). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum tissue with PGP9.5 antibody (clone UCHL1/4558). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PGP9.5 antibody (clone UCHL1/4558) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using PGP9.5 antibody (clone UCHL1/4558). These results demonstrate the foremost specificity of the UCHL1/4558 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human brain tissue and T98G cell lysates using PGP9.5 antibody (clone UCHL1/4558). Predicted molecular weight ~25 kDa.