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PGAM1/PGAM2/PGAM4 Antibody

SKU: orb1247114

Description

PGAM1/PGAM2/PGAM4 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 28.8kDa according to NP_002620.1; NP_000281.2 and of NP_001025062.1). The additional 30kDa band observed in the rodent lysates likely represent the acetylated form. Recommended concentration: 0.05-0.15µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-KAMEAVAAQGKAKK
TargetPGAM1 / PGAM2 / PGAM4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

PGAM1, PGAM2, phosphoglycerate mutase 1 (brain), PGAMA, RP11-452K12.8, OTTHUMP00000059414, Phosphoglycerate mutase A, nonmuscle form, phosphoglycerate mutase 2 (muscle), MGC88743, PGAMM, Phosphoglycerate mutase, muscle form, PGAM3, phosphoglycerate mutase family member 4, PGAM-B, dJ1000K24.1, phosphoglycerate mutase family 3, phosphoglycerate mutase family 4, phosphoglycerate mutase processed protein, bisphosphoglycerate mutase 1, bisphosphoglycerate mutase 2, bisphosphoglycerate mutase 4, BPG-dependent PGAM 1, OTTHUMP00000020190, PGAM-M, BPG-dependent PGAM 2, OTTHUMP00000063546

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PGAM1/PGAM2/PGAM4 Antibody

orb1247114 (0.05 ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig Liver (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PGAM1/PGAM2/PGAM4 Antibody (orb1247114)

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0.1 mg
$ 920.00
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