PFKFB3 Antibody (C-term)

SKU: orb1928562

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD26252
• Immunogen: This PFKFB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-484 amino acids from the C-terminal region of human PFKFB3. Antigen Region: 454-484 aa.
• Target: PFKFB3
• Molecular Weight: 59609 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, 6PF-2-K/Fru-2,6-P2ase 3, PFK/FBPase 3, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, Renal carcinoma antigen NY-REN-56, iPFK-2, 6-phosphofructo-2-kinase, Fructose-2,6-bisphosphatase, PFKFB3

Western blot analysis of anti-PFKFB3 Antibody (C-term) in CEM cell line lysates (35 ug/lane). PFKFB3 (arrow) was detected using the purified Pab.

Western blot analysis of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PFKFB3

UniProt

Q16875

RefSeq (Protein):NP_001138915.1, NP_004557.1