PFKFB3 Antibody (C-term)

• Catalog Number: orb1928562
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PFKFB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-484 amino acids from the C-terminal region of human PFKFB3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q16875
• MW: 59609 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Clone Number: RB04030
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphata
• Note: For research use only
• NCBI: NP_001138915.1, NP_004557.1

Western blot analysis of anti-PFKFB3 Antibody (C-term) in CEM cell line lysates (35 ug/lane). PFKFB3 (arrow) was detected using the purified Pab.

Western blot analysis of lysate from human kidney tissue lysate, using PFKFB3 Antibody (E469). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.