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PFKFB2 Antibody

SKU: orb1538802

Description

PFKFB2 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P
Dilution RangeIHC, IHC-P (2.5 - 3.75 µg/ml), Peptide-ELISA (1:16000)
ReactivityHuman, Monkey
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 75Da band in Human Heart lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.5kDa according to NP_006203.2 and 54.4kDa according to NP_001018063.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence SEQNNNSYETKTP-C, from the N-Terminus of protein sequence according to NP_006203.2NP_001018063.1.
TargetPFKFB2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, PFK-2/FBPase-2, PFK/FBPase 2, PFKFB, cardiac

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Quality Guarantee

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Protocol Information

IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μg
$ 740.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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