PEX3 Antibody

SKU: orb1238074

Description

PEX3 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PEX3 antibody (orb1238074) was raised against a peptide corresponding to 14 amino acids near the center of human PEX3. The immunogen is located within the center of PEX3.
• Target: PEX3
• Molecular Weight: Predicted: 42kDObserved: 42 kD
• Purification: PEX3 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

PEX3 Antibody: Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3

Immunofluorescence Validation of PEX3 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238074, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PEX3 in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX3 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX3 in Rat testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with orb1238074 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Quick Database Links

Gene Symbol

PEX3

UniProt

P56589

RefSeq:P56589