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Item 1 of 7
PEX10 (CT) Antibody
Catalog Number: orb1238073
| Catalog Number | orb1238073 |
|---|---|
| Category | Antibodies |
| Description | PEX10 (CT) Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, WB |
| Predicted Reactivity | Equine, Monkey, Primate |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-PEX10 antibody (orb1238073) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Predicted: 37kD Observed: 37 kD |
| Target | PEX10 |
| UniProt ID | O60683 |
| NCBI | O60683 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PEX10 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
WB Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PEX10 in Human Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX10 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
