PEX10 Antibody

SKU: orb1238073

Description

PEX10 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Monkey, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PEX10 antibody (orb1238073) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10.
• Target: PEX10
• Molecular Weight: Predicted: 37kD Observed: 37 kD
• Purification: PEX10 Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69

WB Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX10 orb1238073, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PEX10 in Human Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of PEX10 in Rat Testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with orb1238073 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Quick Database Links

Gene Symbol

PEX10

UniProt

O60683

RefSeq:O60683