Perp Antibody

• Catalog Number: orb1238785
• Category: Antibodies
• Description: Perp Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-PERP antibody (orb1238785) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human PERP. The immunogen is located within the last 50 amino acids of PERP.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; ICC: 10 μg/mL.Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 21kDObserved: 21 kD
• Target: Perp
• UniProt ID: Q9JK95
• NCBI: NP_071404
• Storage: PERP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PERP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PERP Antibody: THW, KCP1, PIGPC1, KRTCAP1, 1110017
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PERP orb1238785 (1 µg/mL), PERP, orb1268263 (2 µg/mL), beta-actin orb1240312 (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PERP orb1238785 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human A431 whole cell lysates in the Absence (A) and Presence (B) of Blocking Peptide. Loading: 15 µg of lysates per lane. Antibodies: PERP orb1238785 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of PERP in A431 Cells. Immunocytochemical analysis of A431 cells using anti-PEPR antibody (orb1238785) at 10 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.