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PERK (EIF2AK3) Antibody (Center)
Catalog Number: orb1928555
| Catalog Number | orb1928555 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PERK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human EIF2AK3. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | Q9NZJ5 |
| MW | 125216 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:1000, IHC-P: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB12501 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Eukaryotic translation initiation factor 2-alpha k Read more... |
| Note | For research use only |
| NCBI | NP_004827.4 |
All lanes: Anti-EIF2AK3 Antibody (Center) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 125 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EIF2AK3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
