PERK Antibody

• Catalog Number: orb1731537
• Category: Antibodies
• Description: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Recombinant protein of human PERK
• Concentration: 1mg/ml
• Dilution range: WB: 1/500-1/1000
• Conjugation: Unconjugated
• MW: Calculated MW: 125 kDa; Observed MW: 140 kDa
• Entrez: 9451
• UniProt ID: Q9NZJ5
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: EIF2AK3; PEK; PERK; Eukaryotic translation initiat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.