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Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Catalog Number: orb506147

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb506147
CategoryAntibodies
DescriptionPhospho-PERK (Thr980) Rabbit Polyclonal Antibody
TargetEIF2AK3
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Porcine, Rabbit
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980 H(p-T)GQ
UniProt IDQ9Z2B5
MW119 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesp-PERK(Thr980); PERK(phospho Thr980); PERK(phospho
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NoteFor research use only
Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Blank control (Black line): A431 (Black). Primary Antibody (green line): Rabbit Anti-PERK (Thr980) antibody (orb506147), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at -20°C. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-Phospho-PERK (Thr980) antibody (orb506147), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Blank control: A431. Primary Antibody (green line): Rabbit Anti-PERK (Thr980) antibody (orb506147), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 3 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (orb506147) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (orb506147) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (orb506147) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-PERK (Thr980)) Polyclonal Antibody, Unconjugated (orb506147) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-PERK (Thr980) Rabbit Polyclonal Antibody

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human MCF-7-Tg cell lysates, Lane 3: Human MCF-7-phosphatase cell lysates, Primary: Anti-Phospho-PERK (Thr980) (orb506147) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 119 kDa, Observed band size: 120 kDa.

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    FC,  ICC,  IF,  IHC-Fr,  IHC-P

    Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 200 μl, 100 μl
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    Polyclonal

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    100 μl
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    IHC-Fr,  IHC-P

    Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl
  • Phospho-PERK (Thr980) Rabbit Polyclonal Antibody (HRP) [orb504147]

    IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μl
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    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Porcine, Rabbit

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Biotin

    100 μl