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PERK / EIF2AK3

Catalog Number: orb1294328

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb1294328
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PERK
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / encompassing a sequence within the center region
Dilution rangeWestern Blot 1:500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100-1:200
ConjugationUnconjugated
TargetPERK / EIF2AK3
StorageStore at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
NoteFor research use only
Expiration Date12 months from date of receipt.
PERK / EIF2AK3

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK / EIF2AK3

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK / EIF2AK3

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK / EIF2AK3

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294328 Anti-PERK antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

PERK / EIF2AK3

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.

PERK / EIF2AK3

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.

  • Anti-PERK/EIF2AK3 Antibody [orb402265]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • PERK (EIF2AK3) Antibody (Center) [orb1928555]

    IHC-P,  WB

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • PERK (EIF2AK3) Human Over-expression Lysate [orb1346211]

    125.26 kDa

    Human

    100 μg
  • PERK (EIF2AK3) Human Over-expression Lysate [orb1366550]

    125.26 kDa

    Human

    20 μg
  • EIF2AK3 [orb69327]

    ELISA

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μl