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PERK/EIF2AK3 Antibody

Catalog Number: orb1294328

DispatchUsually dispatched within 5-10 working days
$ 260.00
Catalog Numberorb1294328
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PERK
TargetPERK / EIF2AK3
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit-IgG
ConjugationUnconjugated
ReactivityHuman, Mouse
ImmunogenSynthetic peptide / encompassing a sequence within the center region
Tested applicationsIF, IHC, WB
Dilution rangeWestern Blot 1:500 Immunofluorescence 1:300–1:500 Immunohistochemistry (Paraffin) 1:100-1:200
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
NoteFor research use only
PERK/EIF2AK3 Antibody

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK/EIF2AK3 Antibody

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK/EIF2AK3 Antibody

All lanes: Anti-PERK antibody - at 1/500 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294328 overnight at 4°C.

PERK/EIF2AK3 Antibody

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294328 Anti-PERK antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

PERK/EIF2AK3 Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.

PERK/EIF2AK3 Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PERK with orb1294328 at 1/200.

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    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    IHC-P,  WB

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    ELISA

    Human

    Mouse

    Monoclonal

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    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

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    100 μg
  • Anti-PERK/EIF2AK3 Antibody [orb2613055]

    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg