Perforin Antibody [CB5.4]

• Catalog Number: orb1273969
• Category: Antibodies
• Description: Perforin Antibody [CB5.4]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: CB5.4
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.
• Concentration: batch dependent
• Dilution range: Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μg/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: P10820
• Storage: Perforin antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μg/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
• Expiration Date: 12 months from date of receipt.

The immunogen is recombinant mouse perforin amino acids orb1250814. The antibody recognizes an epitope within aa 402-534. Schematic structure of mouse perforin (recombinant, aa. orb1250814) as seen in this image.