Perforin Antibody [CB5.4]

SKU: orb1273969

Description

Perforin Antibody [CB5.4]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Reactivity: Mouse
• Application Notes:
  Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μg/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: CB5.4
• Immunogen: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.
• Purification: Perforin (monoclonal CB5.4) antibody was purified to ≥95% in SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

The immunogen is recombinant mouse perforin amino acids orb1250814. The antibody recognizes an epitope within aa 402-534. Schematic structure of mouse perforin (recombinant, aa. orb1250814) as seen in this image.

Quick Database Links

UniProt

P10820