PEMT Antibody (C-term)

• Catalog Number: orb1939075
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PEMT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UBM1
• MW: 22134 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100
• Antibody Type: Primary Antibody
• Clone Number: RB12628
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phosphatidylethanolamine N-methyltransferase, PEAM
• Note: For research use only
• NCBI: NP_680478.1, NP_009100.2, NP_001254481.1, NP_001254480.1, NP_680477.1

All lanes: Anti-PEMT Antibody (C-term) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: Mouse liver tissue lysate. Lane 4: Mouse ovary tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.