PELP1 Antibody (Center R759)

• Catalog Number: orb1937896
• Category: Antibodies
• Description: PELP1 Antibody (Center R759)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8IZL8
• MW: 119700 Da
• Tested applications: IHC-P, WB
• Dilution range: WB - 1:1000-1:2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HMX3, MNAR
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PELP1 Antibody (Center R759) at 1:1000 dilution. Lane 1: T47D whole cell lysate. Lane 2: mouse testis lysate. Lane 3: Hela whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: SW620 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 120 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining PELP1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Staining PELP1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

PELP1 Antibody (Center R759) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the PELP1 antibody detected the PELP1 protein (arrow).