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Catalog Number | orb1787975 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | 331-358 aa |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Conjugation | Unconjugated |
MW | 43585 Da |
Target | This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI. |
UniProt ID | O75521 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
PECI Antibody (C-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-PECI Antibody (C-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: human heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.