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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | Flow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood);Immunofluorescence (1:50-1:100);Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | JC/70A |
| Immunogen | Membrane preparation of a spleen from a patient with hairy cell leukemia |
| Target | PECAM-1 |
| Source/Expression System | Bioreactor |
| Molecular Weight | ~100kDa (endothelium) and ~130kDa (platelets)' |
| Purification | Protein G |
| Conjugation | CF488 |
Storage & Handling
−| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
|---|---|
| Form/Appearance | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. Contact us if you require this Ab in a different format. |
| Concentration | Purified Ab conjugated to CF488 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
PECAM-1 Antibody (CF488) (orb3073874)
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