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PDX1 Antibody (T11)

Catalog Number: orb1931774

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1931774
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
TargetThis PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityMouse, Rat
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
UniProt IDO00330
MW54122 Da
Tested applicationsIF, IHC-P, WB
Dilution rangeIF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
Antibody TypePrimary Antibody
Clone NumberRB15204
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesPyruvate dehydrogenase protein X component, mitoch
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NoteFor research use only
NCBINP_003468.2, NP_001159630.1, NP_001128496.1
PDX1 Antibody (T11)

Fluorescent confocal image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

PDX1 Antibody (T11)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PDX1 Antibody (T11)

Western blot analysis of anti-PDX1 Antibody (T11) in NCI-H460 cell line lysates (35 ug/lane).PDX1-T11 (arrow) was detected using the purified Pab.

PDX1 Antibody (T11)

Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2).

  • Phospho-PDX1(T11) Antibody [orb1931073]

    DOT,  IF

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Phospho-IPF(T11) Antibody [orb1931088]

    DOT

    Mouse, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • Phospho-PDX1(T11) Antibody [orb1166841]

    DOT,  IF

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 30 μl
  • IPF Antibody (T11) Blocking peptide [orb1446004]

    500 μg
  • PDX1 (Phospho-T11) Antibody Blocking peptide [orb1449796]

    500 μg