PDIp Antibody

• Catalog Number: orb1247127
• Category: Antibodies
• Description: PDIp Antibody
• Target: PDIA2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-NLETTKKYAPVD
• UniProt ID: Q13087
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Pancreas lysates (calculated MW of 58.2kDa according to NP_006840.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine and Heart. Recommended concentration: 3.75ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PDIA2, PDIP, protein disulfide isomerase family A,
• Note: For research use only
• NCBI: NP_006840.2

orb1247127 (0.03 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247127 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247127 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.