PDIA3 Antibody

• Catalog Number: orb626814
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERp57
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: protein disulfide isomerase family A, member 3
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2400; IP: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:20-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: PDIA3
• UniProt ID: P30101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 58 kDa microsomal protein, Disulfide isomerase ER
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of HepG2 cells, using PDIA3 antibody orb626814 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IP Result of anti-ERp57/ERp60 (IP: orb626814, 4 ug; Detection: orb626814 1:2000) with mouse liver tissue lysate 4000 ug.

L02 cells were subjected to SDS PAGE followed by western blot with orb626814 (PDIA3 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human lung cancer using orb626814 (PDIA3 antibody) at dilution of 1:100.