PDI Antibody (HRP)

SKU: orb151396

Description

Rabbit polyclonal to PDI (HRP). The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins. Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins. PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase. PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol. PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus. It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor..

Research Area

Cell Biology, Disease Biomarkers, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Bovine, Canine, Frog, Guinea pig, Hamster, Human, Invertebrate, Mouse, Mussel, Porcine, Rat, Sheep
• Application Notes:
  A 1:1000 dilution was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: AA499-509 of Rat PDI
• Target: PDI
• Molecular Weight: 58kDa
• Purification: Protein A purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Thioredoxin domain containing 5, ERP46, UNQ364, MGC3178, FLJ21353, FLJ90810, thioredoxin related protein, endothelial protein disulphide isomeras

Quick Database Links

Gene Symbol

PDI

UniProt

P04785

NCBI Gene ID

287164

RefSeq (Protein):NP_001099245.2