PDHE1A(S232) Antibody

SKU: orb1925302

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB61811
• Immunogen: This PDHE1A(S232) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 206-237 amino acids from the human region of human PDHE1A(S232).
• Target: PDHA1
• Molecular Weight: 43296 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, 1.2.4.1, PDHE1-A type I, PDHA1, PHE1A

All lanes: Anti-PDHE1A (S232) Antibody at 1:2000 dilution + PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 43 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDHE1A (S232) Antibody at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PDHE1A (S232) Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: RD whole cell lysate. Lane 4: Mouse brain whole lysate. Lane 5: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PDHA1

UniProt

P08559