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PDHA1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499297

Description

PDHA1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

Tested ApplicationsICC, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
ReactivityHuman, Mouse, Rat
Predicted ReactivityMouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.R9B7
ImmunogenA synthesized peptide derived from human PDHA1 (100-150aa)
TargetPDHA1
Molecular Weight43 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; ODPA_HUMAN; EC:1.2.4.1; PDHE1-A type I; pyruvate dehydrogenase E1 subunit alpha 1; PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; PDH-E1α;
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

PDHA1 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: Mouse kidney, 3: Rat, kidney, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 43 kDa, Observed MW: 43 kDa.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

PDHA1 Recombinant Rabbit Monoclonal Antibody (orb1499297)

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Available Sizes

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25 μl
$ 180.00
50 μl
$ 330.00
100 μl
$ 490.00
DispatchUsually dispatched within 5-10 working days
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