PDGFRA / PDGFR Alpha Antibody (aa1000-C-term)

SKU: orb1735048

Description

PDGFRA / PDGFR Alpha Antibody (aa1000-C-term)

Images & Validation

• Tested Applications: IHC, IHC-P, IP, WB
• Dilution range: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 ul immunoprecipitates PDGF Receptor a from 500 ug of 3T3/NIH RIPA lysate.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to C-Terminus of human PDGF Receptor alpha.
• Purification: Immunoaffinity purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
• Disclaimer: For research use only

Alternative Names

PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Western Blot analysis of 3T3/NIH cell lysate using (1:500).