PDGFRA Antibody (Y720)

• Catalog Number: orb1929008
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB11101
• Tested applications: IHC-P, WB
• Predicted Reactivity: Other, Rat
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 122670 Da
• Target: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 amino acids from human PDGFRA.
• UniProt ID: P16234
• NCBI: NP_006197.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Platelet-derived growth factor receptor alpha, PDG
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-PDGFRA Antibody (Y720) at 1:2000 dilution + NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 123 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PDGFRA Antibody (Y720) at 1:1000 dilution + Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 123 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y720), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Staining PDGFRA in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.