PDGFRA Antibody (C-term E1063)

• Catalog Number: orb1929009
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P16234
• MW: 122670 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB2005/2006
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Platelet-derived growth factor receptor alpha, PDG
• Research Area: Cancer Biology, Cardiovascular Research, Infectiou
• Note: For research use only
• NCBI: NP_006197.1
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PDGFRA Antibody (C-term E1063) in K562 cell line lysates (35 ug/lane). PDGFRA (arrow) was detected using the purified Pab.

Western blot analysis of PDGFRA antibody (C-term E1063) in mouse lung tissue lysates (35 ug/lane). PDGFRA (arrow) was detected using the purified Pab.

PDGFRA Antibody (C-term E1063) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.